13432 Cnbp Status: Cancelled
| Gene | Cnbp |
| Chromosome | 6 |
| Deletion Start | 87,845,776 |
| Deletion End | 87,843,964 |
| Deletion Size | 1,813 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,845,498 | 87,845,516 | CGCTGTGGTGAGTCTGGTC |
| TUP | 87,845,470 | 87,845,496 | TCTTGCCAAGGATTGTGATCTGCAGGA |
| TUR | 87,845,429 | 87,845,452 | CACCTCTCCCAAGAGTATCAAGTG |
| TDF | 87,844,105 | 87,844,125 | TGCAGCAAGACAAGTGAAGTC |
| TDP | 87,844,081 | 87,844,101 | TGTTACCGCTGTGGCGAGTCA |
| TDR | 87,844,056 | 87,844,077 | TTGTGCATTCCCGTGCAAGATG |
PCR Primers
| Primer | Sequence |
| SU | CGGATTCTCTGCCACTGTTC |
| SD | GGGCCTGATGCAGATGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
