| Gene | Cnfn |
| Chromosome | 7 |
| Deletion Start | 25,368,471 |
| Deletion End | 25,367,703 |
| Deletion Size | 769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,368,413 | 25,368,432 | TGCTACCAGACACAGCTCAG |
| TUP | 25,368,390 | 25,368,410 | ATTGGCACACGGGCCTCACCG |
| TUR | 25,368,350 | 25,368,369 | TTCCTCGGACACTCACAGAC |
| TDF | 25,367,800 | 25,367,818 | GGGCTGCCCTCACCTTTTG |
| TDP | 25,367,778 | 25,367,798 | CTGCCCTGCGCCCTCTGTCAG |
| TDR | 25,367,723 | 25,367,744 | CAAGGACCAAGGACCAAGAATG |
PCR Primers
| Primer | Sequence |
| SU | CCAGCAAGATGCAGTTTGAG |
| SD | GAGACTGTCTGGGTTGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13433A-A5 | VGB6 | C57BL/6NTac | |
| 13433A-D8 | VGB6 | C57BL/6NTac | |
| 13433A-D12 | VGB6 | C57BL/6NTac | |
| 13433A-F3 | VGB6 | C57BL/6NTac | |
| 13433A-G5 | VGB6 | C57BL/6NTac | |
| 13433A-A2 | VGB6 | C57BL/6NTac | |
