| Gene | Cnpy4 |
| Chromosome | 5 |
| Deletion Start | 138,187,588 |
| Deletion End | 138,192,945 |
| Deletion Size | 5,358 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,188,193 | 138,188,214 | GAAGATGAAGACACCACACCAC |
| TUP | 138,188,219 | 138,188,243 | AAGTTGCCCATCGGTGTGAGGCTTT |
| TUR | 138,188,272 | 138,188,290 | GAGGCCATGCTGCTTGTTG |
| TDF | 138,192,243 | 138,192,264 | TGAGAGGTGTTGAGGGTTTGAG |
| TDP | 138,192,266 | 138,192,290 | CAGGATTATCCCACGTAGCCCAGGC |
| TDR | 138,192,322 | 138,192,346 | CAGTTAGAAGGTAGCGGCAAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGTAGGTCCTGGCAGAAG |
| SD | ACTTTCCCACAGCTCACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13434A-A3 | VGB6 | C57BL/6NTac | |
| 13434B-E8 | VGB6 | C57BL/6NTac | |
