| Gene | Cpsf1 |
| Chromosome | 15 |
| Deletion Start | 76,607,475 |
| Deletion End | 76,595,884 |
| Deletion Size | 11,592 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,607,196 | 76,607,215 | ATCCTACCCTGCCCGGACAC |
| TUP | 76,607,174 | 76,607,194 | TCTGCCCGCGGTCTACTGCTT |
| TUR | 76,607,119 | 76,607,138 | TGCTAAGCTCGTGGAAGGTG |
| TDF | 76,595,958 | 76,595,977 | TCACCACCCTTCCTGTCTAC |
| TDP | 76,595,929 | 76,595,956 | CCTTTACCTCTCCATCACCCACAGATCC |
| TDR | 76,595,907 | 76,595,927 | TCTGTCTCCAGCAAGTCATCC |
PCR Primers
| Primer | Sequence |
| SU | CGTCAGTTCTTATCAAGCTAGGC |
| SD | TGACCCTTCAACCTGTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13442A-A3 | VGB6 | C57BL/6NTac | |
| 13442A-B1 | VGB6 | C57BL/6NTac | |
| 13442A-D2 | VGB6 | C57BL/6NTac | |
| 13442A-D6 | VGB6 | C57BL/6NTac | |
| 13442A-E3 | VGB6 | C57BL/6NTac | |
