13455 Cxcr4 Status: ES cell colonies screened / QC one positive
| Gene | Cxcr4 |
| Chromosome | 1 |
| Deletion Start | 128,592,206 |
| Deletion End | 128,588,772 |
| Deletion Size | 3,435 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,591,446 | 128,591,469 | GTTCCTGAGAAAGGGTCATAAGTC |
| TUP | 128,591,421 | 128,591,445 | TGTACCATGATGCTGCTGGCCATGA |
| TUR | 128,591,390 | 128,591,407 | TCCCAACATGCCCTCCTG |
| TDF | 128,589,692 | 128,589,714 | AGCTAAGGAGCATGACGGACAAG |
| TDP | 128,589,670 | 128,589,691 | TACCGGCTGCACCTGTCAGTGG |
| TDR | 128,589,622 | 128,589,641 | TGGCATCAACTGCCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTCCACCCGTAAACTCCAG |
| SD | TGCAGCAACAGCTGAGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
