13467 Dap Status: ES cell colonies screened / QC no positives
| Gene | Dap |
| Chromosome | 15 |
| Deletion Start | 31,224,532 |
| Deletion End | 31,235,864 |
| Deletion Size | 11,333 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,225,117 | 31,225,140 | CCTCCACTCAGTTCCTGTTTGTTC |
| TUP | 31,225,141 | 31,225,165 | CTCCATGGCTGGAGGAGTTGGCATT |
| TUR | 31,225,188 | 31,225,207 | GGTGCTTCCACAGGTATCTC |
| TDF | 31,235,502 | 31,235,523 | CTTTGTCATGTGGCTTGTGTTC |
| TDP | 31,235,524 | 31,235,551 | TGCTGACGCTGCTGTTCTTAGAAAGTAC |
| TDR | 31,235,563 | 31,235,582 | GCTCCAGTGTTCCATTCTCC |
PCR Primers
| Primer | Sequence |
| SU | GCTACGCTTCCCTCAGTGAC |
| SD | AAGCTATGTGTACCATCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
