13468 Dbi Status: ES cell colonies screened / QC no positives
| Gene | Dbi |
| Chromosome | 1 |
| Deletion Start | 120,120,950 |
| Deletion End | 120,113,436 |
| Deletion Size | 7,515 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,120,145 | 120,120,164 | ATTCCCAGCACTTGACACAG |
| TUP | 120,120,119 | 120,120,144 | TGCTGTAACTGCTTGAGTGGACAGGT |
| TUR | 120,120,077 | 120,120,096 | GCCTCCCATATTCCCTCTTC |
| TDF | 120,113,569 | 120,113,592 | GCACCTTGCCTTTCTAGCATACAG |
| TDP | 120,113,530 | 120,113,559 | TGTGTATTCCTTCCAATTCCTTCCAGGGAC |
| TDR | 120,113,499 | 120,113,522 | CACATAGGTCTTCATGGCACTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCGCATGAGAGTTAAAGGG |
| SD | TCGAGAACCCTGACGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
