| Gene | Dppa3 |
| Chromosome | 6 |
| Deletion Start | 122,626,526 |
| Deletion End | 122,630,009 |
| Deletion Size | 3,484 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,627,322 | 122,627,342 | CTCTGTGTAGCCCAGGTTGTC |
| TUP | 122,627,348 | 122,627,370 | ACGCTGTACACCAGGCTGGCTTG |
| TUR | 122,627,388 | 122,627,410 | TGCACTCGAAAGTCTGAGAAAGG |
| TDF | 122,629,016 | 122,629,035 | GCATCAGCACTGGGTGTTTG |
| TDP | 122,629,040 | 122,629,066 | TTAAGACTCCCTCCCTGTCCAAGCTGG |
| TDR | 122,629,086 | 122,629,103 | GCTCTGAAGAGGCAGGAC |
PCR Primers
| Primer | Sequence |
| SU | AGAGCGGGGAATCCTACAGT |
| SD | CCTGGCAGCAAGCTTTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13484A-H7 | VGB6 | C57BL/6NTac | |
| 13484A-E2 | VGB6 | C57BL/6NTac | |
