Gene | Fastk |
Chromosome | 5 |
Deletion Start | 24,445,144 |
Deletion End | 24,441,152 |
Deletion Size | 3,993 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 24,444,641 | 24,444,662 | GACCACTAGCCCTGTGAATGAC |
TUP | 24,444,617 | 24,444,640 | CTCAGGCTTAACCTCTCCGCCGCT |
TUR | 24,444,554 | 24,444,573 | GTGCAAGCTGATGAGGAGTC |
TDF | 24,441,546 | 24,441,569 | CCAGGCAAACTTTAACTCCACAAC |
TDP | 24,441,521 | 24,441,544 | CAAGACCCTGCCCAAAGGTAGGGA |
TDR | 24,441,469 | 24,441,488 | GGCTCAGGCTGCTGTTAGAC |
PCR Primers
Primer | Sequence |
SU | ATTGGAAACTGGTGGACTGG |
SD | GCACAGCAGAGGGACTAAGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 109 bp | + | + | - |
TDF | TDR | 101 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
13496A-F11 | VGB6 | C57BL/6NTac | |
13496A-B1 | VGB6 | C57BL/6NTac | |
13496A-C2 | VGB6 | C57BL/6NTac | |
13496A-A3 | VGB6 | C57BL/6NTac | |