13496   Fastk     Status: ES cell colonies screened / QC positives

GeneFastk
Chromosome5
Deletion Start24,445,144
Deletion End24,441,152
Deletion Size3,993
Genome Build38
MGI IDMGI:1913837
NCBI Gene ID66587
BAC ID121J9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,444,64124,444,662GACCACTAGCCCTGTGAATGAC
TUP24,444,61724,444,640CTCAGGCTTAACCTCTCCGCCGCT
TUR24,444,55424,444,573GTGCAAGCTGATGAGGAGTC
TDF24,441,54624,441,569CCAGGCAAACTTTAACTCCACAAC
TDP24,441,52124,441,544CAAGACCCTGCCCAAAGGTAGGGA
TDR24,441,46924,441,488GGCTCAGGCTGCTGTTAGAC

PCR Primers

PrimerSequence
SUATTGGAAACTGGTGGACTGG
SDGCACAGCAGAGGGACTAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR109 bp++-
TDFTDR101 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13496A-F11VGB6C57BL/6NTac
13496A-B1VGB6C57BL/6NTac
13496A-C2VGB6C57BL/6NTac
13496A-A3VGB6C57BL/6NTac