| Gene | Foxa2 |
| Chromosome | 2 |
| Deletion Start | 148,045,892 |
| Deletion End | 148,043,469 |
| Deletion Size | 2,424 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,045,390 | 148,045,409 | CGAAGCCGGGACACTAACAG |
| TUP | 148,045,368 | 148,045,389 | TTCCTAGCCGAGTTGGCGGTCC |
| TUR | 148,045,344 | 148,045,364 | GCATCGCGTGGAGGAATAAGG |
| TDF | 148,044,196 | 148,044,213 | TGGCAAGGGCTCCTTCTG |
| TDP | 148,044,171 | 148,044,192 | CCTGCACCCAGACTCGGGCAAC |
| TDR | 148,044,096 | 148,044,116 | GGCTTCCTTCAGTGCCAGTTG |
PCR Primers
| Primer | Sequence |
| SU | CGACACTGCCAGCCTCAG |
| SD | TTTCTCCTGGTCCGGTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13506A-A12 | VGB6 | C57BL/6NTac | |
| 13506A-D9 | VGB6 | C57BL/6NTac | |
