13509 Ros1 Status: ES cell colonies screened / QC one positive
| Gene | Ros1 |
| Chromosome | 10 |
| Deletion Start | 52,194,953 |
| Deletion End | 52,165,344 |
| Deletion Size | 29,610 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,194,918 | 52,194,939 | GCTCACCCTGAAACTTGTGAAG |
| TUP | 52,194,890 | 52,194,915 | TGTGGTCCTTGGGTGCATCATATGGA |
| TUR | 52,194,830 | 52,194,854 | CCAGGTTAGTTACACAGGACGTTAG |
| TDF | 52,165,630 | 52,165,652 | TGGAGGTCATGCAGATAGCAGAG |
| TDP | 52,165,591 | 52,165,616 | CCCAGTGACCCTGAACTGTTCCATGG |
| TDR | 52,165,550 | 52,165,571 | CAGCACCCAGAGACCATCAATC |
PCR Primers
| Primer | Sequence |
| SU | CCTGTGTCAGGATTGTGTGG |
| SD | CAAGAATTTGAAGCCATCTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
