13511 Ftl1 Status: Cancelled
| Gene | Ftl1 |
| Chromosome | 7 |
| Deletion Start | 45,459,628 |
| Deletion End | 45,458,040 |
| Deletion Size | 1,589 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,459,280 | 45,459,303 | GTGTTGCGATTGAAGTTTACAGTG |
| TUP | 45,459,261 | 45,459,279 | TGAGCCGAGCCACCGCGTT |
| TUR | 45,459,226 | 45,459,244 | AAGCCCTGCGGGAGGTTAC |
| TDF | 45,458,292 | 45,458,314 | CGGTGTCTGATGTCTCTTCCTTC |
| TDP | 45,458,261 | 45,458,286 | CAGCTCTGTGACTTCCTGGAAAGCCA |
| TDR | 45,458,199 | 45,458,217 | TGCGGAGGTTGGTCAGATG |
PCR Primers
| Primer | Sequence |
| SU | CCGATTGGTTAGTCTCGGG |
| SD | CTCACCTCCTGGCCTTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
