13521 Gls Status: Cancelled
| Gene | Gls |
| Chromosome | 1 |
| Deletion Start | 52,232,989 |
| Deletion End | 52,207,307 |
| Deletion Size | 25,683 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,232,501 | 52,232,520 | AACGGAGGTTCGCGGAAGAG |
| TUP | 52,232,477 | 52,232,498 | AGAAAGCGATGCCAGGCGGCCT |
| TUR | 52,232,449 | 52,232,469 | GGGCCTAATCACATGGCACAG |
| TDF | 52,207,470 | 52,207,494 | GCATAATTCTGGGTGTGAACAACTG |
| TDP | 52,207,450 | 52,207,468 | TTTGATATGTGCCTTACTC |
| TDR | 52,207,373 | 52,207,396 | TCGTGACAGCATTGTTTCAAATAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCTAGCCAATCAGAAGAG |
| SD | AACTCCTACTGATCTGTAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
