| Gene | Gng8 |
| Chromosome | 7 |
| Deletion Start | 16,894,947 |
| Deletion End | 16,895,448 |
| Deletion Size | 502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,894,994 | 16,895,015 | GCTGAAGCTGGAAGTGAACATC |
| TUP | 16,895,018 | 16,895,045 | TCGCATGAAGGTGCATTAAACCGATGGG |
| TUR | 16,895,065 | 16,895,084 | GCCCTCAACGGAGCCTAATG |
| TDF | 16,895,271 | 16,895,291 | GAAACGCACGCTAAGGATGAC |
| TDP | 16,895,293 | 16,895,315 | CACTGGTGACTCCTGTCCCTGCC |
| TDR | 16,895,319 | 16,895,337 | TGTCGCGGAAGGGATTCTC |
PCR Primers
| Primer | Sequence |
| SU | CTCAGTTCTCCCTGGAGTCG |
| SD | CCCAGGAGGGAATAGAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13527A-B6 | VGB6 | C57BL/6NTac | |
| 13527A-G7 | VGB6 | C57BL/6NTac | |
