| Gene | Gpatch4 |
| Chromosome | 3 |
| Deletion Start | 88,051,214 |
| Deletion End | 88,055,763 |
| Deletion Size | 4,550 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,051,601 | 88,051,619 | GCGGTTAGCCCAGGAAGAC |
| TUP | 88,051,624 | 88,051,646 | TGAGGGCTCAGGTCAGGCTACAA |
| TUR | 88,051,654 | 88,051,675 | TGCCTGTAGAGGAGAGTGAGTG |
| TDF | 88,055,376 | 88,055,398 | GGAGTCTGTGATGAAGGAGGTAG |
| TDP | 88,055,401 | 88,055,424 | ATGTGACAAGCCGGCCAAAGGCAG |
| TDR | 88,055,462 | 88,055,481 | CACACGTCCCTCGCTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | CCTGAGCTCCAAACTCAATC |
| SD | ATCAGAACAGTCTGGGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13531A-A4 | VGB6 | C57BL/6NTac | |
| 13531A-A6 | VGB6 | C57BL/6NTac | |
| 13531A-D9 | VGB6 | C57BL/6NTac | |
| 13531A-F5 | VGB6 | C57BL/6NTac | |
| 13531A-G1 | VGB6 | C57BL/6NTac | |
