13579 Id3 Status: ES cell colonies screened / QC no positives
| Gene | Id3 |
| Chromosome | 4 |
| Deletion Start | 136,143,882 |
| Deletion End | 136,144,418 |
| Deletion Size | 537 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,143,993 | 136,144,013 | AGCCTCTTGGACGACATGAAC |
| TUP | 136,144,019 | 136,144,040 | CTACTCGCGCCTGCGGGAACTG |
| TUR | 136,144,116 | 136,144,134 | CTGCCAGGACCACCTGAAG |
| TDF | 136,144,227 | 136,144,245 | GCTGCGGGTCTTCCCTATC |
| TDP | 136,144,251 | 136,144,274 | CCCGAGTCCCTTGGCTAACTCGTC |
| TDR | 136,144,312 | 136,144,334 | TTGTCCTTGGAGATCACAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGGACCTCAGAGAACTTGGG |
| SD | TCCAGCTGATAACTCCGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 142 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
