13597 Kcnu1 Status: ES cell colonies screened / QC no positives
| Gene | Kcnu1 |
| Chromosome | 8 |
| Deletion Start | 25,849,659 |
| Deletion End | 25,881,458 |
| Deletion Size | 31,800 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,849,682 | 25,849,705 | AAATCAGAAGGAGTTGACGGAAAC |
| TUP | 25,849,707 | 25,849,734 | TCATGTACAATCGAAATCCAGGCAGCGT |
| TUR | 25,849,736 | 25,849,756 | GTCGCCAAGGAGGAAAGAATG |
| TDF | 25,881,083 | 25,881,100 | GGGTGCCTGCCACATTTC |
| TDP | 25,881,102 | 25,881,131 | CCAGCAGATGTCAATGCTAATCACGATGGC |
| TDR | 25,881,141 | 25,881,160 | TCGAGTGGGAACCAGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | AAAGCTTGAAATTGCTTGGC |
| SD | TCCCTCCTCTTCAGAAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
