13598 Klf2 Status: ES cell colonies screened / QC no positives
| Gene | Klf2 |
| Chromosome | 8 |
| Deletion Start | 72,319,081 |
| Deletion End | 72,321,011 |
| Deletion Size | 1,931 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,319,227 | 72,319,245 | GACAAGGTCCAGGGTGCTG |
| TUP | 72,319,248 | 72,319,272 | CGCCTCAGGGAGTTAGACTTCAGGC |
| TUR | 72,319,314 | 72,319,331 | TGCACGGCATCCCTTCAG |
| TDF | 72,320,580 | 72,320,600 | ATGCACCTGAGCCTGCTAGTG |
| TDP | 72,320,602 | 72,320,624 | TCAGCTTCAGGAGCCCAAGGTTC |
| TDR | 72,320,633 | 72,320,657 | TCTCATCTCCATCCCACATCTAATC |
PCR Primers
| Primer | Sequence |
| SU | GCGCGCTAACTATGCTGTTG |
| SD | CGTGATTCCTCCAAAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
