13599 Klf4 Status: ES cell colonies screened / QC no positives
| Gene | Klf4 |
| Chromosome | 4 |
| Deletion Start | 55,531,725 |
| Deletion End | 55,528,059 |
| Deletion Size | 3,667 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,531,095 | 55,531,112 | GAGGCTGCCTTCCTGTTC |
| TUP | 55,531,068 | 55,531,094 | TGTCCAGTTCCTATAGCAGGGTGTTGG |
| TUR | 55,531,047 | 55,531,066 | TGACTTGTTGGGTGAAGCAC |
| TDF | 55,528,310 | 55,528,329 | TCAGCAGCCCGTTCTCTTTC |
| TDP | 55,528,276 | 55,528,303 | CAGGCGAGAAACCTTACCACTGTGACTG |
| TDR | 55,528,223 | 55,528,244 | GGTAGTGCCTGGTCAGTTCATC |
PCR Primers
| Primer | Sequence |
| SU | AAAGAAGAAGGATCTCGGGC |
| SD | GAAGGCAAAGAGCATACAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
