| Gene | Lat |
| Chromosome | 7 |
| Deletion Start | 126,369,247 |
| Deletion End | 126,364,122 |
| Deletion Size | 5,126 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,369,116 | 126,369,137 | AGCTTTGCGGAACTGGATGATG |
| TUP | 126,369,092 | 126,369,113 | TGGGCGCTCCATTGGATCCTCA |
| TUR | 126,369,052 | 126,369,071 | CAGGTAGGGACAGTGGAGAG |
| TDF | 126,364,925 | 126,364,945 | GCCACTTCTTCACAGATCACC |
| TDP | 126,364,895 | 126,364,918 | TCTGCACTCTGAGCTCCTTGGAGC |
| TDR | 126,364,846 | 126,364,869 | AATGGAACAGAGACAATACAGGAC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGCCTGATGATTTCCTG |
| SD | GGCTGGAACCAGATTCATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13604A-H5 | VGB6 | C57BL/6NTac | |
| 13604B-E10 | VGB6 | C57BL/6NTac | |
