13610 Lrp8 Status: ES cell colonies screened / QC no positives
| Gene | Lrp8 |
| Chromosome | 4 |
| Deletion Start | 107,802,416 |
| Deletion End | 107,803,366 |
| Deletion Size | 951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,802,766 | 107,802,786 | GGGTAGTCCTTCAGGGTCTGG |
| TUP | 107,802,791 | 107,802,815 | TGGTTTAATTTGACACGCGCGCTCC |
| TUR | 107,802,851 | 107,802,869 | TCCCCGGAAACGCATTGTC |
| TDF | 107,803,250 | 107,803,268 | GGGCCAGTCAAGGAGTGTG |
| TDP | 107,803,275 | 107,803,298 | ACCAGTTTCGGTGTCGGAACGAGC |
| TDR | 107,803,325 | 107,803,345 | CGAGCAGTCGTTGTCCTCATC |
PCR Primers
| Primer | Sequence |
| SU | AGCGTCACCGAACCTGCTT |
| SD | TGCGTGGAACTTTGAAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
