13622 Matn2 Status: Cancelled
| Gene | Matn2 |
| Chromosome | 15 |
| Deletion Start | 34,406,745 |
| Deletion End | 34,435,784 |
| Deletion Size | 29,040 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,316,981 | 34,317,001 | CACCTGTCTGAAGGGCCTATG |
| TUP | 34,317,005 | 34,317,031 | TAACAGCTGCCTGCAAGCTATGAAGGT |
| TUR | 34,317,038 | 34,317,061 | ACAACCCTGAACGGTCTCTGAAAC |
| TDF | 34,344,566 | 34,344,587 | GGCAGAGGGAATGAGGAAGAAG |
| TDP | 34,344,590 | 34,344,611 | TGAAGCCTTCCAGCACCGTGCT |
| TDR | 34,344,627 | 34,344,651 | AGCATGGTGAGTTTGGCAAGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCAGCTGCACATTGATTTC |
| SD | ACATGGCCTAGATTAGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
