13627 Mesp2 Status: Cancelled
| Gene | Mesp2 |
| Chromosome | 7 |
| Deletion Start | 79,810,932 |
| Deletion End | 79,812,852 |
| Deletion Size | 1,921 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,811,620 | 79,811,639 | CCCTAGAAAGAGCCGCTGAC |
| TUP | 79,811,642 | 79,811,663 | CTCTCCCTGGGCACCACCTCAA |
| TUR | 79,811,699 | 79,811,720 | TGTCCAGTGTCCAGTCTTGTTC |
| TDF | 79,812,521 | 79,812,542 | TCTTGCAGAGTCTTTCTGTGTC |
| TDP | 79,812,544 | 79,812,565 | CCAGAACCCTGCCTGTCCCTGG |
| TDR | 79,812,602 | 79,812,620 | TGAGGCTGAGGCTGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | GACATCCAGGTACCCAGCAG |
| SD | TGCAGAGTGCTGAGCCTTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
