13643 Mrpl34 Status: ES cell colonies screened / QC no positives
| Gene | Mrpl34 |
| Chromosome | 8 |
| Deletion Start | 71,465,006 |
| Deletion End | 71,465,469 |
| Deletion Size | 464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,465,041 | 71,465,061 | CAGATCGGTGGCCCTATTGAG |
| TUP | 71,465,062 | 71,465,087 | TGGCAGGTGAGTGTTCAGAGATACCC |
| TUR | 71,465,095 | 71,465,116 | GAGACCACCCTTGTTTCCATTC |
| TDF | 71,465,337 | 71,465,358 | CGAGTATCAGCCGAGCAACATC |
| TDP | 71,465,359 | 71,465,381 | AAGCGCAAGCACAAGCACGGCTG |
| TDR | 71,465,434 | 71,465,453 | AGGGACTTGCGGCCCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCGAGGCGAAGAACTACAAC |
| SD | ACTTTGGAATGGCTGGAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 117 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
