13645 Mrps23 Status: ES cell colonies screened / QC no positives
| Gene | Mrps23 |
| Chromosome | 11 |
| Deletion Start | 88,204,445 |
| Deletion End | 88,210,814 |
| Deletion Size | 6,370 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,204,601 | 88,204,621 | TCGGTGTGGTTTGTCTCTGTC |
| TUP | 88,204,623 | 88,204,651 | CCTTGGATTTCTGTCCTGTCAGATTGTGC |
| TUR | 88,204,684 | 88,204,704 | CAGGCCCTTCAGCTTCTTTAC |
| TDF | 88,210,625 | 88,210,647 | TCACATTCTCTGCAAACCCTCAC |
| TDP | 88,210,651 | 88,210,676 | TCAGCGTGAGACTCCAAGCTTCATCA |
| TDR | 88,210,695 | 88,210,713 | GCGCCAGGTCATCGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTTAGTCGAGCCTGGTGTC |
| SD | ACTGGGTGTGATCTTCGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
