13647 Msln Status: ES cell colonies screened / QC no positives
| Gene | Msln |
| Chromosome | 17 |
| Deletion Start | 25,754,216 |
| Deletion End | 25,748,669 |
| Deletion Size | 5,548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,753,580 | 25,753,600 | GCCCTGAAAGGAGCTGAGTTG |
| TUP | 25,753,550 | 25,753,576 | ACAGTGGAGCCCTTGGGTGTTAAGTCC |
| TUR | 25,753,499 | 25,753,520 | TCTGAGAGCAAGGTCCAGATAC |
| TDF | 25,749,029 | 25,749,051 | CCAAACATTGTGGACCTGAAGAC |
| TDP | 25,748,992 | 25,749,017 | AAAGCCCTGTCCGTGACTGGCTGTTC |
| TDR | 25,748,942 | 25,748,961 | CCACCCTGAAGTCCCAAACC |
PCR Primers
| Primer | Sequence |
| SU | TGAACCACTCAGTGCTCCTG |
| SD | GGCGGATCTGAATAAAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
