13649 Msx2 Status: Parental BAC obtained
| Gene | Msx2 |
| Chromosome | 13 |
| Deletion Start | 53,568,098 |
| Deletion End | 53,563,541 |
| Deletion Size | 4,558 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,567,390 | 53,567,410 | GTTGAGTCAGGGTCTCCTGTG |
| TUP | 53,567,365 | 53,567,387 | CCGTGCTGGTATGCAGCTCAGGA |
| TUR | 53,567,335 | 53,567,357 | TGGTGGAGTAAGATGACGAGTTC |
| TDF | 53,563,927 | 53,563,946 | CACCTGCACCCTGAGGAAAC |
| TDP | 53,563,903 | 53,563,926 | ACAAGACCAACCGGAAGCCACGCA |
| TDR | 53,563,871 | 53,563,892 | CAAGGCTAGAAGCTGGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | TTGGGCGATTATAACCCTTG |
| SD | AAGCAAGTCCACCATCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
