13651 Mtmr10 Status: ES cell colonies screened / QC no positives
| Gene | Mtmr10 |
| Chromosome | 7 |
| Deletion Start | 64,287,785 |
| Deletion End | 64,318,342 |
| Deletion Size | 30,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,287,951 | 64,287,970 | GCCATTCTCTGGCCGAAGTC |
| TUP | 64,287,972 | 64,287,991 | TGCGGTCCCTAGTCCTGCCG |
| TUR | 64,287,998 | 64,288,017 | CCGGACGGTTCTACGATCTC |
| TDF | 64,318,239 | 64,318,261 | GGATTCTGCTTGTCTCCTAGTTC |
| TDP | 64,318,265 | 64,318,287 | TGCTGGAGCCACTCTAACGGCAG |
| TDR | 64,318,309 | 64,318,326 | TGCAGCGCGTCTTTGATG |
PCR Primers
| Primer | Sequence |
| SU | AAGCGCGCTCTCAAGATGG |
| SD | ACAGGGAGGTTACTGCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
