13659 Ndrg2 Status: ES cell colonies screened / QC no positives
| Gene | Ndrg2 |
| Chromosome | 14 |
| Deletion Start | 51,911,537 |
| Deletion End | 51,906,044 |
| Deletion Size | 5,494 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,911,505 | 51,911,524 | AGGTGCAGATCACCGAGGAG |
| TUP | 51,911,481 | 51,911,504 | AAGCCGCTGTTGCCAGGACAAACA |
| TUR | 51,911,455 | 51,911,477 | TCTCTGTAAACCTTGGCAGTCTC |
| TDF | 51,906,738 | 51,906,759 | ATCCTTCTTCCCTGGGTGTTTC |
| TDP | 51,906,714 | 51,906,736 | TTCCTGAGCAGATGGCCGACTCT |
| TDR | 51,906,666 | 51,906,687 | TCCCAAGATGGGCACTATACTC |
PCR Primers
| Primer | Sequence |
| SU | GTTTGGTATTGCCCTGGAAG |
| SD | CCTTCAGAGAGGCCTGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
