13665 Nfkbia Status: Cancelled
| Gene | Nfkbia |
| Chromosome | 12 |
| Deletion Start | 55,492,523 |
| Deletion End | 55,489,817 |
| Deletion Size | 2,707 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,491,757 | 55,491,777 | TTCCTGCACTTGGCAATCATC |
| TUP | 55,491,732 | 55,491,756 | CACGAAGAGAAGCCGCTGACCATGG |
| TUR | 55,491,698 | 55,491,717 | AAGGCCAGGTCTCCCTTCAC |
| TDF | 55,490,792 | 55,490,812 | GTGGAGCACTTGGTGACTTTG |
| TDP | 55,490,764 | 55,490,789 | TGCTGATGTCAACGCTCAGGTGAGTA |
| TDR | 55,490,728 | 55,490,749 | GAGCCAGCCCAACAGAGATTAG |
PCR Primers
| Primer | Sequence |
| SU | AGAAGGGTTCTTGCAGAGGG |
| SD | AAGCACATGCCACTGAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
