13666 Ngp Status: ES cell colonies screened / QC no positives
| Gene | Ngp |
| Chromosome | 9 |
| Deletion Start | 110,419,865 |
| Deletion End | 110,422,702 |
| Deletion Size | 2,838 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,419,979 | 110,419,997 | CAAGGGCGGCAAGGAAGAC |
| TUP | 110,419,999 | 110,420,021 | CCTCTTCCGCCTGCTAAGTGCCA |
| TUR | 110,420,039 | 110,420,063 | CCAGATTGGAATGGCAGATACTCAC |
| TDF | 110,422,141 | 110,422,163 | CGTGACTCATCATGCCCTTGTAG |
| TDP | 110,422,165 | 110,422,191 | TGATACCTCTGTTGCCTCACCCTCTTC |
| TDR | 110,422,199 | 110,422,217 | CAACCCGCCTGTGGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGAAGAGCCAGGTAGACCG |
| SD | GCCTCACAAGGTGATCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
