13676 Noxo1 Status: ES cell colonies screened / QC no positives
| Gene | Noxo1 |
| Chromosome | 17 |
| Deletion Start | 24,698,346 |
| Deletion End | 24,700,422 |
| Deletion Size | 2,077 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,699,071 | 24,699,093 | GCTTCTTTGTACCCAAACCTCTG |
| TUP | 24,699,095 | 24,699,117 | ATCTGGAGCCCATGCTGCCTCCT |
| TUR | 24,699,155 | 24,699,176 | TCATCTGACCCTCCAACAAGTG |
| TDF | 24,699,726 | 24,699,747 | ACACCCAGGAGGGAGGTAGTAG |
| TDP | 24,699,753 | 24,699,778 | CACTAGGCATGTGGGTGCTGCTTAGA |
| TDR | 24,699,800 | 24,699,820 | CACCCTGCACTGCTCTGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GGTGGATTCAAAGAGCAAGC |
| SD | TCCTCTAGAGCGGCACTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
