13681 Nr0b2 Status: ES cell colonies screened / QC one positive
| Gene | Nr0b2 |
| Chromosome | 4 |
| Deletion Start | 133,553,428 |
| Deletion End | 133,556,274 |
| Deletion Size | 2,847 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,554,182 | 133,554,200 | GCCAAGCTAGTGCAGAGAC |
| TUP | 133,554,201 | 133,554,221 | TGCTCTTTGCCACCGTGCCTG |
| TUR | 133,554,223 | 133,554,247 | CTCCCTAAGATTCAAGCCACATTTC |
| TDF | 133,555,846 | 133,555,868 | CAAGGTGGTAGAGTGAGCCAATG |
| TDP | 133,555,877 | 133,555,902 | TCATTAGTGAAGGATCGCCTCAGTGC |
| TDR | 133,555,914 | 133,555,933 | TGTGAGGGTGTCAGCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGTCATCCGATAAAGGGC |
| SD | CTGGCTTCACAGTCAGTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
