| Gene | Nrn1l |
| Chromosome | 8 |
| Deletion Start | 105,893,583 |
| Deletion End | 105,894,966 |
| Deletion Size | 1,384 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,893,689 | 105,893,707 | CCAGGCTGCAAACCCTCTC |
| TUP | 105,893,713 | 105,893,738 | TCCTAGCCAGACTGGGTGTAGGGTAT |
| TUR | 105,893,749 | 105,893,770 | CCTCACTGCTCATCTCCAACTC |
| TDF | 105,894,351 | 105,894,370 | TCTCTACCCACCCAGCAGTG |
| TDP | 105,894,373 | 105,894,394 | CTCTGAGGGCCCAAACCGCTGT |
| TDR | 105,894,412 | 105,894,434 | GGCGGATGAGACATTCAGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCCTGGCACTCATTCTCATC |
| SD | ATGGAGAACTCTCACCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13683A-B1 | VGB6 | C57BL/6NTac | |
| 13683A-B4 | VGB6 | C57BL/6NTac | |
| 13683A-C7 | VGB6 | C57BL/6NTac | |
| 13683A-D10 | VGB6 | C57BL/6NTac | |
| 13683A-E4 | VGB6 | C57BL/6NTac | |
