| Gene | Nsg2 |
| Chromosome | 11 |
| Deletion Start | 32,001,755 |
| Deletion End | 32,017,729 |
| Deletion Size | 15,975 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,001,802 | 32,001,822 | CGTGGAGGATGGTTTCCAGAC |
| TUP | 32,001,825 | 32,001,846 | TCCCTCTCATCACGCCCTTGGA |
| TUR | 32,001,881 | 32,001,903 | GGAAAGGAAGATCACCCGGTTAC |
| TDF | 32,017,429 | 32,017,450 | AATACCCATAACGCTGGCAAAC |
| TDP | 32,017,453 | 32,017,478 | AATCGGAGAGGCAGAATCAGCAGCGT |
| TDR | 32,017,498 | 32,017,521 | TTCAAATAGCCCATAGCACTCTCC |
PCR Primers
| Primer | Sequence |
| SU | AGAACAATCTTGCCAGTGGG |
| SD | ATTTCCTGAAGATTGTGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13684A-G10 | VGB6 | C57BL/6NTac | |
| 13684A-C5 | VGB6 | C57BL/6NTac | |
| 13684A-G1 | VGB6 | C57BL/6NTac | |
| 13684A-E10 | VGB6 | C57BL/6NTac | |
