| Gene | Oaz1 |
| Chromosome | 10 |
| Deletion Start | 80,826,763 |
| Deletion End | 80,829,049 |
| Deletion Size | 2,287 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,827,303 | 80,827,323 | GGTCTGCCTCTCGGATATGTG |
| TUP | 80,827,324 | 80,827,353 | AGCTAGTTCTAATGTCAGGTGGCGAAGTGT |
| TUR | 80,827,364 | 80,827,388 | GATTTATTACCCAAACGCCCTACAG |
| TDF | 80,828,140 | 80,828,157 | GCAGTGACTCGGGTTGAC |
| TDP | 80,828,161 | 80,828,186 | TGTCAATGGCTCTTGAGTGGGAAGCC |
| TDR | 80,828,214 | 80,828,234 | GCAGGAAACAATGCTCAGGTG |
PCR Primers
| Primer | Sequence |
| SU | ACGGAAACCTTCTGTCTGTC |
| SD | TACCTCAGCCACTTTCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13695A-A9 | VGB6 | C57BL/6NTac | |
| 13695A-B10 | VGB6 | C57BL/6NTac | |
| 13695A-C1 | VGB6 | C57BL/6NTac | |
| 13695A-F10 | VGB6 | C57BL/6NTac | |
| 13695A-E12 | VGB6 | C57BL/6NTac | |
| 13695A-D6 | VGB6 | C57BL/6NTac | |
