13701 Pabpn1 Status: ES cell colonies screened / QC no positives
| Gene | Pabpn1 |
| Chromosome | 14 |
| Deletion Start | 54,894,159 |
| Deletion End | 54,898,204 |
| Deletion Size | 4,046 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,894,825 | 54,894,847 | CATGCCCTGCTCATTCATTGTAG |
| TUP | 54,894,853 | 54,894,878 | AGAAGCGATCAAAGCTCGAGTCAGGG |
| TUR | 54,894,880 | 54,894,899 | TCTCAGCCTCTTCCTCCATC |
| TDF | 54,897,345 | 54,897,365 | GACCCTTACTCCCTTGTCCTC |
| TDP | 54,897,366 | 54,897,389 | CCTTTGGTTGCAGGGACTTGGTCC |
| TDR | 54,897,400 | 54,897,421 | GCCTGCCACTATTTCCTTACTC |
PCR Primers
| Primer | Sequence |
| SU | TCGAATCCCATAAGCCAATC |
| SD | AAAAGGCAGGGAGAAGACGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
