13702 Padi2 Status: ES cell colonies screened / QC no positives
| Gene | Padi2 |
| Chromosome | 4 |
| Deletion Start | 140,906,437 |
| Deletion End | 140,934,716 |
| Deletion Size | 28,280 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,907,311 | 140,907,332 | AGCCTGAGAGGCCACACTGAAG |
| TUP | 140,907,334 | 140,907,357 | CACCATCTGCTCCGAGCACTCTCA |
| TUR | 140,907,358 | 140,907,375 | CCTCGCAAGGCAGAACAC |
| TDF | 140,934,051 | 140,934,068 | GCTCCTTTGCCTGCCATC |
| TDP | 140,934,069 | 140,934,097 | TCCCTCTGTTTGCTTTAATCTTTGAGCCA |
| TDR | 140,934,098 | 140,934,117 | CCTGGGCTACAACGTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | TATTCACTCCTGCCCTGTCC |
| SD | GCAGACTTCTGTCTTGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
