13703 Pax2 Status: ES cell colonies screened / QC no positives
| Gene | Pax2 |
| Chromosome | 19 |
| Deletion Start | 44,757,397 |
| Deletion End | 44,788,857 |
| Deletion Size | 31,461 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,757,516 | 44,757,533 | TCACCCTAGAGGCCACAC |
| TUP | 44,757,534 | 44,757,557 | TGGCCTTTCCCTGCTCCCTCCTTT |
| TUR | 44,757,578 | 44,757,596 | GGCTGAAGGCGTGAGAAAC |
| TDF | 44,788,721 | 44,788,739 | TGTGGCATGTGGCTTGTGG |
| TDP | 44,788,744 | 44,788,769 | TGGCCTGTGACAATGTTGTGGATGCT |
| TDR | 44,788,793 | 44,788,811 | GGTGGAAAGGCTGCTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGCTGAGTGAGCCGTCTTC |
| SD | AGTAAACATGAAAGAGCGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
