13710 Pde11a Status: ES cell colonies screened / QC no positives
| Gene | Pde11a |
| Chromosome | 2 |
| Deletion Start | 76,338,604 |
| Deletion End | 76,337,702 |
| Deletion Size | 903 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,338,242 | 76,338,262 | GCTTCGCAGAAAGAGCTGAGG |
| TUP | 76,338,214 | 76,338,235 | TTTGCCCGCTCCAAAGCCATCC |
| TUR | 76,338,177 | 76,338,196 | CGGGAGGTCACCTGTTCATC |
| TDF | 76,337,956 | 76,337,977 | GACATCTCCAATGACCTTGACC |
| TDP | 76,337,927 | 76,337,955 | TCACCAGCCTAAGCTACAAGATCCTCATC |
| TDR | 76,337,904 | 76,337,923 | GCGTCCACCATGAGACAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTCAGAGCTTCACTCC |
| SD | CACAGATCCACTCTAAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
