13715 Peo1 Status: ES cell colonies screened / QC no positives
| Gene | Peo1 |
| Chromosome | 19 |
| Deletion Start | 45,007,133 |
| Deletion End | 45,011,977 |
| Deletion Size | 4,845 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,007,578 | 45,007,599 | AGGTTAGGGAGGAGGTCTTGAG |
| TUP | 45,007,602 | 45,007,628 | TCTGGAACCGAGCCATACCTCTTTGGG |
| TUR | 45,007,666 | 45,007,687 | CACCTTGGTAAGGCCAAACATC |
| TDF | 45,011,674 | 45,011,695 | CGCTTTGATGGAGACGTAGGTG |
| TDP | 45,011,699 | 45,011,725 | TCCCACTGGAGTTCAACAAGAATTCCC |
| TDR | 45,011,744 | 45,011,763 | GAGTCGGGCTTTGCTCTTGG |
PCR Primers
| Primer | Sequence |
| SU | AGGTTAGCCAGGAGAGTCCG |
| SD | CCCTTTCTGCAGAGGTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
