| Gene | Pou4f3 |
| Chromosome | 18 |
| Deletion Start | 42,394,646 |
| Deletion End | 42,396,089 |
| Deletion Size | 1,444 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,395,188 | 42,395,207 | CGTCTCCCACGGCAAGAATC |
| TUP | 42,395,209 | 42,395,228 | TCCGTTCAAGCCCGACGCCA |
| TUR | 42,395,229 | 42,395,250 | ACGCTGCTCATGGTATGGTAGG |
| TDF | 42,395,799 | 42,395,818 | TCAACGGCAGTGAGCGTAAG |
| TDP | 42,395,819 | 42,395,839 | CGCAAACGCACGTCCATCGCC |
| TDR | 42,395,865 | 42,395,884 | TGGCTGGATGGCGAAATAGG |
PCR Primers
| Primer | Sequence |
| SU | ATGCAAAGAAGTCCAGGAGG |
| SD | TTCGCCCTTGCCTAATAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13731A-F9 | VGB6 | C57BL/6NTac | |
| 13731A-G6 | VGB6 | C57BL/6NTac | |
| 13731A-H7 | VGB6 | C57BL/6NTac | |
