13736 Ppp1cb Status: ES cell colonies screened / QC no positives
| Gene | Ppp1cb |
| Chromosome | 5 |
| Deletion Start | 32,459,359 |
| Deletion End | 32,491,142 |
| Deletion Size | 31,784 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,459,724 | 32,459,743 | CTCTTTGTGCGTTGCCCTTG |
| TUP | 32,459,745 | 32,459,767 | CAGCCTCCGATGGTCGTTCCGGT |
| TUR | 32,459,792 | 32,459,814 | CAAACGATCCGAGAGCCTTACTG |
| TDF | 32,490,263 | 32,490,282 | CATGCCTTTGATCCCAGCAG |
| TDP | 32,490,284 | 32,490,311 | CGGGAGCAGATCTCTGAGTTCAAAGCTA |
| TDR | 32,490,346 | 32,490,369 | GCAACAAGGTTCCTCTGTGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGAGGAGGTGGAGGAGG |
| SD | CATGCTTTCATCTGGGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
