13747 Ptms Status: ES cell colonies screened / QC no positives
| Gene | Ptms |
| Chromosome | 6 |
| Deletion Start | 124,917,601 |
| Deletion End | 124,914,129 |
| Deletion Size | 3,473 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,917,178 | 124,917,199 | TGCCACTATGACCTCACCTTTC |
| TUP | 124,917,152 | 124,917,176 | TCTGCTAAGCGGTTTGGGATCCTCA |
| TUR | 124,917,130 | 124,917,150 | CCGGGAGAGTCCTGTTTACAC |
| TDF | 124,914,346 | 124,914,364 | AAGGGCTGGGTGGCCTTTG |
| TDP | 124,914,320 | 124,914,342 | TTGGACCCAGGTCCTTGTGGGTA |
| TDR | 124,914,220 | 124,914,240 | GGTCTGCTTCATCCTGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTAAAAGCGGGTTCCCTTC |
| SD | GAGGGAATCTGAGGGAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 145 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
