13756   Rabac1     Status: ES cell colonies screened / QC positives

GeneRabac1
Chromosome7
Deletion Start24,972,579
Deletion End24,969,919
Deletion Size2,661
Genome Build38
MGI IDMGI:1201692
NCBI Gene ID14470
BAC ID373A15
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,971,88224,971,900GCAGCCTCGGATACCTAAC
TUP24,971,85624,971,880CCTGTCTCCCACACTCTTCCAGGGT
TUR24,971,82024,971,839AAGACAGCCAGAGCCACCAG
TDF24,970,20224,970,221TGGGAACGGCTACCTTACAC
TDP24,970,18024,970,201CATCTGTTCCCAGGCCGGGAGG
TDR24,970,14824,970,168ACCAGCCAGGGCATACTGATG

PCR Primers

PrimerSequence
SUTGCTGTAGTCCACGCTGAG
SDCCCTCTCCTTACCTATAAAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR81 bp++-
TDFTDR74 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13756A-D10VGB6C57BL/6NTac
13756A-E4VGB6C57BL/6NTac
13756A-E8VGB6C57BL/6NTac
13756A-H3VGB6C57BL/6NTac
13756A-G3VGB6C57BL/6NTac
13756A-E1VGB6C57BL/6NTac