| Gene | Recql4 |
| Chromosome | 15 |
| Deletion Start | 76,710,473 |
| Deletion End | 76,703,720 |
| Deletion Size | 6,754 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,709,742 | 76,709,760 | GTGGAGGTGAACCCAGAAC |
| TUP | 76,709,716 | 76,709,739 | CCTTTGGCCCTTGGGTGGTTTGCC |
| TUR | 76,709,691 | 76,709,712 | GGAAGGGTGGACATAGAAGGAC |
| TDF | 76,703,836 | 76,703,853 | ACCCAGCCCAGGTGTATG |
| TDP | 76,703,805 | 76,703,829 | ACCGGCGCTTCTGGAGGAAGTACCT |
| TDR | 76,703,776 | 76,703,795 | GGTGCATCAGGGCATGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGTGCCCTGACCACTAACG |
| SD | CTTGTACTCTCAGCCCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13766A-H7 | VGB6 | C57BL/6NTac | |
| 13766A-H6 | VGB6 | C57BL/6NTac | |
| 13766A-G2 | VGB6 | C57BL/6NTac | |
| 13766A-E9 | VGB6 | C57BL/6NTac | |
| 13766A-D12 | VGB6 | C57BL/6NTac | |
| 13766A-E1 | VGB6 | C57BL/6NTac | |
