| Gene | Rhbdf1 |
| Chromosome | 11 |
| Deletion Start | 32,216,255 |
| Deletion End | 32,209,709 |
| Deletion Size | 6,547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,215,726 | 32,215,744 | TCCGTCTGCCTCTCAGTTC |
| TUP | 32,215,694 | 32,215,721 | AGTATGCACCCTCCCTACTCTAACACCC |
| TUR | 32,215,657 | 32,215,676 | ACACGCCCAAAGCGTACCTG |
| TDF | 32,210,065 | 32,210,082 | CTGCCCTGGATCGACAAC |
| TDP | 32,210,042 | 32,210,064 | TTCGCCCACATCTCGGGCTTTGT |
| TDR | 32,210,013 | 32,210,034 | GGAAGGCAAAGGAAAGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTTCACTTGGGAGCTGTC |
| SD | GCCTTCCTAGTGCTGTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13772A-B3 | VGB6 | C57BL/6NTac | |
| 13772A-H4 | VGB6 | C57BL/6NTac | |
| 13772A-D2 | VGB6 | C57BL/6NTac | |
| 13772A-G6 | VGB6 | C57BL/6NTac | |
| 13772A-E3 | VGB6 | C57BL/6NTac | |
