13773 Ropn1 Status: ES cell colonies screened / QC no positives
| Gene | Ropn1 |
| Chromosome | 16 |
| Deletion Start | 34,678,534 |
| Deletion End | 34,666,699 |
| Deletion Size | 11,836 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,667,051 | 34,667,068 | AGCACATGCCCGCCTAAC |
| TUP | 34,667,070 | 34,667,094 | CATCCCTGCATCCCATCTGCTTTCC |
| TUR | 34,667,105 | 34,667,126 | TGCCAGCCAGATTCCTAAACTC |
| TDF | 34,678,426 | 34,678,449 | GATGGCTTAATCAAGGTGAATGAC |
| TDP | 34,678,462 | 34,678,489 | CCTAGGGTTCGGCTGGAATAAAAGCACA |
| TDR | 34,678,507 | 34,678,527 | ACAGCCACCTCTGTATCTTCC |
PCR Primers
| Primer | Sequence |
| SU | CACACAGGCTATTGTTTCCG |
| SD | AGCAGCGGACTAGTGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
