13775   Rhov     Status: ES cell colonies screened / QC no positives

GeneRhov
Chromosome2
Deletion Start119,271,120
Deletion End119,270,041
Deletion Size1,080
Genome Build38
MGI IDMGI:2444227
NCBI Gene ID228543
BAC ID210K13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF119,270,759119,270,780GCTGTGCCTAAGTCATGCTTTC
TUP119,270,730119,270,753CCCACCTCTGCAGTGCAAGTCCTG
TUR119,270,662119,270,681GGCTACACCCAGGTTCTCAC
TDF119,270,186119,270,203TGAGTGCTCGGCCTTGAC
TDP119,270,150119,270,177ACTTGAAGGAGGTGTTCGACTCGGCCAT
TDR119,270,113119,270,133CTTCTCCAGACGGGCTTTGTG

PCR Primers

PrimerSequence
SUCCGGTCTTTGTCCTTGTACC
SDCTGTTCTCCTTGATCCTCGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR119 bp++-
TDFTDR91 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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