13779 Rnf139 Status: Cancelled
| Gene | Rnf139 |
| Chromosome | 15 |
| Deletion Start | 58,889,317 |
| Deletion End | 58,900,235 |
| Deletion Size | 10,919 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,889,414 | 58,889,433 | TCGACGCCATCTTCAACTCC |
| TUP | 58,889,435 | 58,889,458 | ACTACGATTCCAGCCAAAGCCGGT |
| TUR | 58,889,495 | 58,889,513 | AGCCCTACGCCGTCCTTAC |
| TDF | 58,899,796 | 58,899,819 | TCTGCTCGTATAACACCGTGTAAC |
| TDP | 58,899,826 | 58,899,848 | TTCCATGCACTCTGCCTTCGGAA |
| TDR | 58,899,865 | 58,899,887 | TGATGGCACATTGGACAAGTATC |
PCR Primers
| Primer | Sequence |
| SU | TCAAGATGGTGGCTCCGCT |
| SD | TTCTAGCAGGTTATCTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
